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人抗胰島素受體抗體酶免試劑盒,(AIRA)ELISA檢測試劑盒 ,英文名:AIRA ELISA Kit ,英文縮寫:AIRA ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗胰島素受體抗體ELISA檢測試劑盒,AIRA 檢測試劑盒, AIRA 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗胰島素受體抗體酶免試劑盒,(AIRA)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
淀粉樣蛋白β1-40(Aβ1-40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)48T/96TY08485Mus musculus (Mouse)
淀粉樣蛋白β1-40(Aβ1-40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)48T/96TY08486Rattus norvegicus (Rat)
熱休克蛋白β2(HSPβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08487Mus musculus (Mouse)
熱休克蛋白β2(HSPβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08488Rattus norvegicus (Rat)
熱休克蛋白β2(HSPβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein Beta 2 (HSPb2)48T/96TY08489Oryctolagus cuniculus (Rabbit)
熱休克蛋白40(HSP40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96TY08490Rattus norvegicus (Rat)
熱休克蛋白40(HSP40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96TY08491Mus musculus (Mouse)
熱休克蛋白40(HSP40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 40 (HSP40)48T/96TY08492Xenopus (Clawed frog)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08493Oryctolagus cuniculus (Rabbit)
70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)48T/96TY08494Sus scrofa; Porcine (Pig)
90kDa熱休克蛋白β1(HSP90β1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Beta 1 (HSP90b1)48T/96TY08495Sus scrofa; Porcine (Pig)
脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY08496Homo sapiens (Human)
脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY08497Rattus norvegicus (Rat)
脂蛋白關(guān)聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Lipoprotein Associated (LpPLA2)48T/96TY08498Mus musculus (Mouse)
內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08499Homo sapiens (Human)
內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08500Rattus norvegicus (Rat)
內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY08501Mus musculus (Mouse)
誘導(dǎo)型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY08502Rattus norvegicus (Rat)
誘導(dǎo)型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY08503Mus musculus (Mouse)
誘導(dǎo)型一氧化氮合酶(NOS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 2, Inducible (NOS2)48T/96TY08504Oryctolagus cuniculus (Rabbit)
一氧化氮合酶運輸因子(NOSTRIN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN)48T/96TY08505Rattus norvegicus (Rat)
一氧化氮合酶運輸因子(NOSTRIN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase Trafficker (NOSTRIN)48T/96TY08506Mus musculus (Mouse)
巨噬細(xì)胞移動抑制因子(MIF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Migration Inhibitory Factor (MIF)48T/96TY08507Homo sapiens (Human)
趨化因子C-C-基元受體8(CCR8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-C-Motif Receptor 8 (CCR8)48T/96TY08508Mus musculus (Mouse)
免疫球蛋白G1(IgG1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G1 (IgG1)48T/96TY08509Homo sapiens (Human)
環(huán)磷酸鳥苷(cGMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclic Guanosine Monophosphate (cGMP)48T/96TY08510General
腫瘤蛋白p53(TP53)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY08511Homo sapiens (Human)
腫瘤蛋白p53(TP53)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY08512Mus musculus (Mouse)
腫瘤蛋白p53(TP53)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY08513Rattus norvegicus (Rat)
P物質(zhì)(SP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Substance P (SP)48T/96TY08514Rattus norvegicus (Rat)
P物質(zhì)(SP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Substance P (SP)48T/96TY08515Mus musculus (Mouse)
速激肽受體1(TACR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tachykinin Receptor 1 (TACR1)48T/96TY08516Homo sapiens (Human)
速激肽受體1(TACR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tachykinin Receptor 1 (TACR1)48T/96TY08517Rattus norvegicus (Rat)
速激肽受體1(TACR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tachykinin Receptor 1 (TACR1)48T/96TY08518Mus musculus (Mouse)
5-羥色胺(5-HT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 5-Hydroxytryptamine (5-HT)48T/96TY08519General
γ-氨基丁酸(γABA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gamma-Aminobutyric Acid (gABA)48T/96TY08520General
多巴胺受體D4(DRD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08521Homo sapiens (Human)
多巴胺受體D4(DRD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08522Rattus norvegicus (Rat)
α2-纖溶酶抑制因子(α2PI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-2-Plasmin Inhibitor (a2PI)48T/96TY08523Rattus norvegicus (Rat)
多巴胺受體D4(DRD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08524Mus musculus (Mouse)
多巴胺受體D4(DRD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D4 (DRD4)48T/96TY08525Canis familiaris; Canine (Dog)
M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Kinase, Muscle (PKM2)48T/96TY08526Homo sapiens (Human)
M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Kinase, Muscle (PKM2)48T/96TY08527Rattus norvegicus (Rat)
M2-型丙酮酸激酶(PKM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Kinase, Muscle (PKM2)48T/96TY08528Mus musculus (Mouse)
凝血因子Ⅱ(F2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor II (F2)48T/96TY08529Rattus norvegicus (Rat)
凝血因子Ⅱ(F2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Coagulation Factor II (F2)48T/96TY08530Mus musculus (Mouse)
前動力蛋白受體2(PKR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prokineticin Receptor 2 (PKR2)48T/96TY08531Homo sapiens (Human)
牛血清白蛋白(BSA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bovine Serum Albumin (BSA)48T/96TY08532General
免疫球蛋白G3(IgG3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin G3 (IgG3)48T/96TY08533Mus musculus (Mouse)
干擾素α(IFNα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY08534Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。