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小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒,英文名:MMP-2/Gelatinase A ELISA Kit,英文縮寫:MMP-2/Gelatinase A ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠基質(zhì)金屬蛋白酶2/明膠酶AELISA檢測試劑盒,MMP-2/Gelatinase A 檢測試劑盒,MMP-2/Gelatinase A 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠基質(zhì)金屬蛋白酶2/明膠酶A酶免試劑盒,(MMP-2/Gelatinase A)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競爭法及定量檢測影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
組織金屬蛋白酶抑制因子2(TIMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY11535Chicken (Gallus)
組織金屬蛋白酶抑制因子2(TIMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY11536Cavia (Guinea pig )
組織金屬蛋白酶抑制因子2(TIMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY11537Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子2(TIMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY11538Oryctolagus cuniculus (Rabbit)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11539Bos taurus; Bovine (Cattle)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11540Capra hircus; Caprine (Goat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11541Equus caballus; Equine (Horse)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11542Danio rerio (Zebrafish)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11543Chicken (Gallus)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11544Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11545Oryctolagus cuniculus (Rabbit)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY11546Rhesus monkey (Simian)
粘蛋白6(MUC6)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Mucin 6 (MUC6)48T/96TY11547Homo sapiens (Human)
突觸足蛋白(SYNPO)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Synaptopodin (SYNPO)48T/96TY11548Homo sapiens (Human)
胰島素樣生長因子結(jié)合蛋白2(IGFBP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY11549Bos taurus; Bovine (Cattle)
胰島素樣生長因子結(jié)合蛋白2(IGFBP2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY11550Sus scrofa; Porcine (Pig)
雙調(diào)蛋白(AREG)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Amphiregulin (AREG)48T/96TY11551Oryctolagus cuniculus (Rabbit)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11552Bos taurus; Bovine (Cattle)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11553Canis familiaris; Canine (Dog)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11554Capra hircus; Caprine (Goat)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11555Equus caballus; Equine (Horse)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11556Chicken (Gallus)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11557Mus musculus (Mouse)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11558Sus scrofa; Porcine (Pig)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11559Oryctolagus cuniculus (Rabbit)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY11560Rhesus monkey (Simian)
轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11561Bos taurus; Bovine (Cattle)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11562Canis familiaris; Canine (Dog)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11563Capra hircus; Caprine (Goat)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11564Equus caballus; Equine (Horse)
轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11565Danio rerio (Zebrafish)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11566Chicken (Gallus)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11567Cavia (Guinea pig )
轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11568Sus scrofa; Porcine (Pig)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11569Rattus norvegicus (Rat)
轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)48T/96TY11570Oryctolagus cuniculus (Rabbit)
轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Transforming Growth Factor Beta 3 (TGFb3)48T/96TY11571Rhesus monkey (Simian)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11572Bos taurus; Bovine (Cattle)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11573Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY11574Oryctolagus cuniculus (Rabbit)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY11575Capra hircus; Caprine (Goat)
腫瘤壞死因子α(TNFα)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)48T/96TY11576Equus caballus; Equine (Horse)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11577Bos taurus; Bovine (Cattle)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11578Canis familiaris; Canine (Dog)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11579Sus scrofa; Porcine (Pig)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11580Oryctolagus cuniculus (Rabbit)
腫瘤壞死因子β(TNFβ)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY11581Rhesus monkey (Simian)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11582Bos taurus; Bovine (Cattle)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11583Capra hircus; Caprine (Goat)
胰島素樣生長因子結(jié)合蛋白3(IGFBP3)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY11584Equus caballus; Equine (Horse)
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。