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大鼠血小板衍生生長因子BB酶免試劑盒,(PDGF-BB)ELISA檢測試劑盒 ,英文名:PDGF-BB ELISA Kit ,英文縮寫:PDGF-BB ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:大鼠血小板衍生生長因子BBELISA檢測試劑盒,PDGF-BB 檢測試劑盒, PDGF-BB 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。大鼠血小板衍生生長因子BB酶免試劑盒,(PDGF-BB)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對競爭法及定量檢測影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性 HOPC-os cDNA人少突膠質(zhì)前體細(xì)胞(懸浮生長)cDNAY3103020 reactions
HSC cDNA人雪旺細(xì)胞cDNAY3103120 reactions
HPC cDNA人神經(jīng)束膜細(xì)胞cDNAY3103220 reactions
HA cDNA人星形膠質(zhì)細(xì)胞cDNAY3103320 reactions
HA-c cDNA人小腦星形膠質(zhì)細(xì)胞cDNAY3103420 reactions
HA-sp cDNA人脊髓星形膠質(zhì)細(xì)胞cDNAY3103520 reactions
HA-h cDNA人海馬趾星形膠質(zhì)細(xì)胞cDNAY3103620 reactions
HM cDNA人小膠質(zhì)細(xì)胞cDNAY3103720 reactions
HDMEC cDNA人真皮微血管內(nèi)皮細(xì)胞cDNAY3103820 reactions
HDLEC cDNA人真皮血管平滑肌細(xì)胞cDNAY3103920 reactions
HEK cDNA人表皮角質(zhì)細(xì)胞cDNAY3104020 reactions
HEK-a cDNA人表皮角質(zhì)細(xì)胞-成人cDNAY3104120 reactions
HEM-l cDNA人表皮黑色素細(xì)胞-淺色素cDNAY3104220 reactions
HEM-m cDNA人表皮黑色素細(xì)胞-中色素cDNAY3104320 reactions
HEM-d cDNA人表皮黑色素細(xì)胞-深色素cDNAY3104420 reactions
HDF-f cDNA人真皮成纖維細(xì)胞-胎兒cDNAY3104520 reactions
HDF-n cDNA人真皮成纖維細(xì)胞-新生兒cDNAY3104620 reactions
HDF-a cDNA人真皮成纖維細(xì)胞-成人cDNAY3104720 reactions
HHDPC cDNA人毛乳頭細(xì)胞cDNAY3104820 reactions
HHGMC cDNA人生發(fā)基質(zhì)細(xì)胞cDNAY3104920 reactions
HHORSC cDNA人毛囊外根殼細(xì)胞cDNAY3105020 reactions
HHIRSC cDNA人毛囊內(nèi)根殼細(xì)胞cDNAY3105120 reactions
HLEC cDNA人淋巴內(nèi)皮細(xì)胞cDNAY3105220 reactions
HLF cDNA人淋巴成纖維細(xì)胞cDNAY3105320 reactions
HOK cDNA人口腔角質(zhì)細(xì)胞cDNAY3105420 reactions
HGF cDNA人牙齦成纖維細(xì)胞cDNAY3105520 reactions
HPLF cDNA人牙周膜成纖維細(xì)胞cDNAY3105620 reactions
HEEpiC cDNA人食道上皮細(xì)胞cDNAY3105720 reactions
HESMC cDNA人食道平滑肌細(xì)胞cDNAY3105820 reactions
HIMEC cDNA人腸微血管內(nèi)皮細(xì)胞cDNAY3105920 reactions
HISMC cDNA人腸平滑肌細(xì)胞cDNAY3106020 reactions
HCSMC cDNA人結(jié)腸平滑肌細(xì)胞cDNAY3106120 reactions
HPMEC cDNA人肺微血管內(nèi)皮細(xì)胞cDNAY3106220 reactions
HPAEC cDNA人肺動(dòng)脈內(nèi)皮細(xì)胞cDNAY3106320 reactions
HPASMC cDNA人肺動(dòng)脈平滑肌細(xì)胞cDNAY3106420 reactions
HPAF cDNA人肺動(dòng)脈成纖維細(xì)胞cDNAY3106520 reactions
HPAEpiC cDNA人肺泡上皮細(xì)胞cDNAY3106620 reactions
HBEpiC cDNA人支氣管上皮細(xì)胞cDNAY3106720 reactions
HTEpiC cDNA人氣管上皮細(xì)胞cDNAY3106820 reactions
HSAEpiC cDNA人小氣道上皮細(xì)胞cDNAY3106920 reactions
HPF cDNA人肺成纖維細(xì)胞cDNAY3107020 reactions
HBSMC cDNA人支氣管平滑肌細(xì)胞cDNAY3107120 reactions
HTSMC cDNA人氣管平滑肌細(xì)胞cDNAY3107220 reactions
HSkMC cDNA人骨骼肌細(xì)胞cDNAY3107320 reactions
HSkMSC cDNA人骨骼肌衛(wèi)星細(xì)胞cDNAY3107420 reactions
HSkMM cDNA人骨骼肌成肌細(xì)胞cDNAY3107520 reactions
HRGEC cDNA人腎小球內(nèi)皮細(xì)胞cDNAY3107620 reactions
HRPTEpiC cDNA人腎近曲小管上皮細(xì)胞cDNAY3107720 reactions
HRCEpiC cDNA人腎皮質(zhì)上皮細(xì)胞cDNAY3107820 reactions
HREpiC cDNA人腎上皮細(xì)胞cDNAY3107920 reactions
各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。